Journal: Journal of visualized experiments : JoVE

Article Title: Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

doi: 10.3791/62913

Figure Lengend Snippet:

Article Snippet: 12 , CMs Cell Culture Medium Complete , Celprogen Inc , M11041 –14S , CMs culture complete medium.

Techniques: Isolation, Cell Counting, Cell Culture, Sterility, Co-Culture Assay, Passaging, CyQUANT Assay, Microscopy, Saline

Journal: Journal of visualized experiments : JoVE

Article Title: Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

doi: 10.3791/62913

Figure Lengend Snippet:

Article Snippet: 15 , CMs Hypoxia Cell Culture Medium , Celprogen Inc , M11041 –14GFPN , CMs cell culture under hypoxic condition (glucose- and serum-free).

Techniques: Isolation, Cell Counting, Cell Culture, Sterility, Co-Culture Assay, Passaging, CyQUANT Assay, Microscopy, Saline

Journal: Journal of visualized experiments : JoVE

Article Title: Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

doi: 10.3791/62913

Figure Lengend Snippet:

Article Snippet: 11 , CMs Cell Culture Flasks T25 , Celprogen Inc , E11041 –14 , Used for CMs regular culture coated by manufactary.

Techniques: Isolation, Cell Counting, Cell Culture, Sterility, Co-Culture Assay, Passaging, CyQUANT Assay, Microscopy, Saline

Journal: Journal of visualized experiments : JoVE

Article Title: Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

doi: 10.3791/62913

Figure Lengend Snippet:

Article Snippet: 13 , CMs Cell Culture Medium Complete Phenol free , Celprogen Inc , M11041 –14PN , CMs culture medium without phenol red used during LDH measurement.

Techniques: Isolation, Cell Counting, Cell Culture, Sterility, Co-Culture Assay, Passaging, CyQUANT Assay, Microscopy, Saline

Journal: Pharmacology Research & Perspectives

Article Title: Poloxamer 188 Protects Isolated Adult Mouse Cardiomyocytes from Reoxygenation Injury

doi: 10.1002/prp2.639

Figure Lengend Snippet: A, Representative photo of the general appearance of the isolated adult mouse cardiomyocytes 6 h after plating. At least 50% of the cells show the expected rod‐shaped morphology of cardiomyocytes. B, Representative photo of the typical appearance of the cardiomyocytes ~ 72 h after plating. A confluent layer has formed, with the cells becoming more rounded due to increased cell‐cell contact and limited growth area. Scale bars are 100 µm in length

Article Snippet: Isolated cardiomyocytes from ventricles of adult male C57BL/6J mouse hearts, as well as the culture media, passaging solutions, and flasks and plates precoated with a proprietary laminin‐based extracellular matrix for cell adherence, were obtained from Celprogen (Torrance, CA, USA).

Techniques: Isolation